Are your cell lines protected from existing patents?
Intellectual property (IP) can be your best friend or your worst nightmare. Are you confident that your future drug was developed with a cell line that is free of any IP claims and that patent owners have no claims to a piece of your future revenue?
Are you using aequorin?
Are you aware that PerkinElmer holds an exclusive license* to the aequorin technology from the University of Georgia? If you are using or looking to use this amazing fully-validated photoprotein in calcium flux luminescence applications, make sure that you have clear IP and work directly with PerkinElmer.
*Patent restrictions in the United States. |
If you obtain cell lines or other materials from unlicensed suppliers, the patent owner has the option of seeking a court injunction to prohibit you from the further use of the unlicensed materials. If unlicensed materials are used in or used to develop a product, you could be prevented from selling that product until the patent expires.
Alternatively, the patent owner may allow you to continue your activities for a fee he/she dictates. In a worst case scenario, the patent owner may prohibit you from continuing your activities, and seek money from you for the activities that you did perform with the unlicensed materials.
What does PerkinElmer do to protect you?
At PerkinElmer, we protect our customers by purchasing applicable licenses from patent holders for our targets. All cell lines go through regularly scheduled and extensive intellectual property searches to provide the most accurate updates in the ever changing IP landscape. Should we fail to obtain rights to a product, we respect the rights of patent owners by restricting our commercial activities to territories where we know there will be no IP infringement. Although these restrictions can sometimes be confusing for customers, the restrictions show our respect for intellectual property rights and are intended to protect our customers and their activities. PerkinElmer provides added security to its customers through indemnification language in its terms and conditions. Through this language, PerkinElmer promises to defend its customers if a patent owner makes a claim against the customer’s use of a PerkinElmer product. In contrast, many other cell line suppliers offer terms and conditions that expressly transfer the legal/IP obligation to the customer, with some suppliers requiring the customers to even protect the supplier in a case of a claim from a patent owner.
Over 300 IP-protected GPCR cell lines and frozen cells available
PerkinElmer offers over 300 GPCR and ion channel cell lines validated for many applications. Our validated, ready-to-use frozen cells remove the hassel associated with routine cell line maintenance and development, offering rapid, worry-free results. Added to this is the assurance that every PerkinElmer cell line is free and clear of patent restrictions.
Announcing the addition of 17 new stable GPCR cell lines and 46 ready-to-use frozen cells
PerkinElmer continues to expand the depth of its cell line and frozen cell portfolio to simplify discovery, profiling and screening campaigns. Our cell lines typically have saturation and competition binding data along with at least one functional assay (agonist/antagonist dose response curve using reference ligands) enabling you to obtain immediate results (Figures below). AequoZen® and cAMPZen® frozen cells have been validated in cAMP and aequorin assays respectively.
| Cell Line |
Validation Assay |
| ValiScreen® |
cAMP, GTPγS, fluorescence calcium, electrophysiology or membrane potential |
| AequoScreen®/ PhotoScreen® |
Fluorescence calcium, aequorin or Photina® luminescence calcium |
Agonist Response in LANCE® cAMP Assays (CCR6 cAMPZen data [ES-139-CF])
An agonist dose-response experiment was performed in 384-well format using 2,500 cells/well and 10 µM forskolin. Reader: EnVision®. Data from a representative experiment are shown.
Agonist/Antagonist Dose Response in AequoScreen Assay (Edg AequoScreen data [ES-594-A])
An agonist dose-response experiment (Top) was performed in a 96-well format using 5,000 cells/well. Luminescence was measured using the MicroBeta®Jet luminescence counter. The antagonist dose response (Bottom) was performed in the same format using 23 nM Sphingosine-1-phosphate (reference agonist).
Thaw and go with frozen cells
Especially convenient for "assaying on demand", PerkinElmer’s validated AequoZen and cAMPZen frozen cell products delink tedious cell culture from your high throughput screening and are available for a wide range of targets. It is no longer necessary to grow cells —simply thaw a vial and use the cells in cAMP, aequorin, fluorescent calcium or ERK assay.
Assay Window and Consistency of EC50 Values for AequoScreen (cell line) and AequoZen (frozen cells)
The correlation between EC50 values of the reference agonist obtained on fresh vs. frozen cells with a calcium aequorin readout on 15 receptors (Top). The pharmacology comparison between fresh AequoScreen 5-HT6 cells natural coupling Gas and Ga16 and equivalent frozen cells (Bottom). |
